1578 pT7A3-RecQ
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Cloning of 1578 pT7A3-RecQR
[edit]Purpose: Cloning of1578 pT7A3-RecQ1==
Date: 2014-7----
Notebook:0
Page:
Clone ID:1578
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from MG1655
digest with Nde1 + XhoI, clone to 1892
Colony Screening
[edit]Date: 2014_7
Make miniprep, use Nde1 + XhoI to find positive clone
USE EcorRV to verify
sent 1578-4 to sequence, not right
Cloning of 1578 pT7A3-RecQR
[edit]Purpose: Cloning of1578 pT7A3-RecQ1==
Date: 2014-8----
Notebook:0
Page:
Clone ID:1578
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from MG1655
digest with Nde1 + XhoI, clone to 1892
Colony Screening
[edit]Date: 2014_8
Make miniprep, use Nde1 + XhoI to find positive clone
