1619-pET28b-T4DNAPol
Cloning of 1619-pET28b-T4DNAPol
[edit]Purpose: Cloning of 1619-pET28b-T4DNAPol
Date: 2013-3-25---2013-3-29
Notebook:0
Page:
Clone ID:1619
Experimenters: yingying
Results
[edit]cloning
[edit]
lane1: PCR product
lane2: pET28b vector
Colony Screening
[edit]Date: 2013-3-29
Make miniprep and digest with Nhe1 + Not1
Result intepretation/Summary
[edit]Conclusion
[edit]Cloning of 1619-pET28b-T4DNAPol
[edit]Purpose: Fix of Cloning of 1619-pET28b-T4DNAPol
Date: 2013-4-29--2013-5-1
Notebook:0
Page:
Clone ID:1619
Experimenters: yingying
Results
[edit]cloning
[edit]
template:1619-4 above
pcr1primer: 1-1 to 3-8
pcr2 primer: 3-9 to 3-24
template: pcr1+pcr2
primer: 1-1 to 3-24
clone PCR product to pET28b
Colony Screening
[edit]Date: 2013-5-1
Make miniprep and digest Nhe1 + Not1
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]
fix of Cloning of 1619-pET28b-T4DNAPol
[edit]Purpose: Fix of Cloning of 1619-pET28b-T4DNAPol
Date: 2013-5-6---2013-5-23
Notebook:0
Page:
Clone ID:1619
Experimenters: yingying
Results
[edit]cloning
[edit]
template:1619-1 (above2013/5/1)
pcr1 primer: 1-1 to 2-10
pcr2 primer: 2-11 to 3-24
template: pcr1+pcr2
primer: 1-1 to 3-24
1: vector pET28b
Nhe1 + Not1
Colony Screening
[edit]Date: 2013-5-23
Make miniprep and digest Nhe1 + Not1
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]fix of Cloning of 1619-pET28b-T4DNAPol
[edit]Purpose: fix of Cloning of 1519-pT7A3-T3-DNA-Ligase
Date: 2013-6-18---2013-6-24
Notebook:0
Page:
Clone ID:1619
Experimenters: yingying
Results
[edit]cloning
[edit]template:1619-1 (above2013/5/1)
pcr1 primer: 1-1 to 2-10
pcr2 primer: 2-11 to 3-24
template: pcr1+pcr2
primer: 1-1 to 3-24
lane 1:digest PCR product
lane 2: pET28b
Nhe+Not1
Colony Screening
[edit]Date: 2013-6-24
Make miniprep and digest with Nhe+Not1
Result intepretation/Summary
[edit]Conclusion
[edit]fix of Cloning of 1619-pET28b-T4DNAPol
[edit]Purpose: fix of Cloning of 1519-pT7A3-T3-DNA-Ligase
Date: 2013-6-24---2013-6-27
Notebook:0
Page:
Clone ID:1619
Experimenters: yingying
Results
[edit]cloning
[edit]template:1619-1 (above2013/5/1)
pcr1 primer: 1-1 to 2-10
pcr2 primer: 2-11 to 3-24
template: pcr1+pcr2
primer: 1-1 to 3-24
lane 3:digest PCR product
lane 4: pET28b
Nhe+Not1
Colony Screening
[edit]Date: 2013-6-27
Make miniprep and digest with Nhe+Not1
Comment by Paul: do not use expensive enzyme such as Not1 for screening
fix of Cloning of 1619-pET28b-T4DNAPol
[edit]Purpose: fix of Cloning of 1619-pT7A3-T3-DNA-Ligase
Date: 2013-7
Notebook:05846
Page:
Clone ID:1619
Experimenters:Shanyi
Results
[edit]cloning
[edit]template:1619-1 (above2013/5/1)
pcr1 primer: 1-1 to 2-10
pcr2 primer: 2-11 to 3-24
template: pcr1+pcr2
primer: 1-1 to 3-24
digest with Nhe+Not1
Colony Screening
[edit]Date: 2013-6-27
Make miniprep and digest with HindIII
