1631 1693 1898 1934 1924 1926 1942 1995 2001

== Protein Expression == 2014/1/24
SHANYI |

Lane	Name	Protein Name	Clone ID	Host	Induction	Purification
1	PageRuler Plus Prestained protein ladder	10,15,*25,35,55,*70,100,130,250
2	pT7A3-ID3	inhibitor of DNA binding 3	1631	5 ml, Nico21(DE3)plysN	0.2 mM IPTG  RT o/n	Ni beads	5.1/13.5	grow slowly
3	pT7A3-8H-LambdaPP	Lambda Protein Phosphatase	1693	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	6.08 / 26.6	cell almost dead
4	pT7A3-8H-Bst-Exo-	thermostable Bacillus DNA polymerase I large fragment	1898	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.86 / 69	grow slowly,low expression
5	pT7A3-6H-sso7d-Bst1-Exo	sso7d-Bst1-Exo	1934	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	6.16 / 76.7	grow slowly,no expression
6	pT7A3E-phi29e-HI	phi29e-HI	1924	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	8.39 / 80.0	low expression
7	pT7A3-phi29wt-sso7d	phi29wt-sso7d	1926	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	8.70 / 75.7	yes
8	pT7A3-new-phi29	new Phi29	1942	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.72/70.6	yes
9	pT3A3-Kfu-sso7d	Kfu-sso7d	1995	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	7.87 / 99.3	yes
10	pT7A3E-HC-EcoSSB	Ecoli SSB	2001	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	6.23 / 20.7	yes