169-pET28b-ssfGFP

Cloning of 169-pET28b-ssfGFP

Purpose: Cloning of 169-pET28b-ssfGFP by gene synthesis

Date: 2011-12-28

Notebook:

Page:

Clone ID:169

Experimenters: yingying

Results

gene synthesis, mkate1 + ssfGFP19, using these mixture: ssfGFP1
ssfGFP2
ssfGFP3
ssfGFP4
ssfGFP5
ssfGFP6
ssfGFP7
ssfGFP8
ssfGFP9
ssfGFP10
ssfGFP11
ssfGFP12
ssfGFP13
ssfGFP14
ssfGFP15
ssfGFP16
ssfGFP17
ssfGFP18
ssfGFP19

Primer-F mkate1, 2010_07_07_Paul Xu_5909785_3602904.XLS Primer-R ssfGFP19

	band 1	band 2
	PCR product	pET28b
digest enzyme	BamH1 + Xho1

subcloning

Date: 

Colony Screening

Date: 2012-1-3

Make miniprep and digest with BamH1 + Xho1

clone 1,3,4,5,6 may be right, need to sequence

 

Result intepretation/Summary

• Sequencing Result:
  

Conclusion

--Yingying.lu 16:03, 29 December 2011 (EST)