1708 2116 2115 2107Taq-muts
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2014/3/
| lane |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 | ||||
| clone ID |
1708 pT7A3-HTNCre |
2116 pT7A3-Penetratin-Cre |
2115 pT7A3-iFLP |
PageRuler Plus Prestained protein ladder |
2115
pT7A3-iFLP |
2107 taq-muts | ||||||||||
| host |
Nico21DE3plysN |
Nico21DE3plysN |
BL21(DE3)Star pRare2pLysS |
Nico21(DE3) pRare2pLysS |
Tuner(de3) |
Nico21DE3plysN | ||||||||||
| induction |
0.1 mM IPTG 25 oC o/n 5ml |
0.1mM IPTG 25 oC o/n 5ml |
N | |||||||||||||
| purification |
Ni beads |
Ni beads |
Ni column | |||||||||||||
| MW |
10.30 / 42.8 |
pI/Mw: 9.76 / 52.8 |
10,15, 25, 35, 55, 70, 100, 130, 250 |
9.76 / 52.8 |
5.67 / 92.8 | |||||||||||