1720 1757 1759 1796 1800 1803 1804 1809 1759 MBP-Keap1 1719

8/26/2013

Lane	Clone ID	Desc	Induction	Purification	MW, KDa	Expression
1	1720	pT7A3-MBP-(E3C)-Keap1, MBP-(E3C)-Keap1-6XHis	0.2 mM IPTG, 25 oC o/n	Ni	116	Yes
2	1757	pT7A3-MBP-(3C)-FN3K N-terminus His Tag	0.2 mM IPTG, 25 oC o/n	Ni	81	weak
3	1759	pCold-6H-(TEV)-MBP-(3C)-Keap1 N-terminus His Tag	0.2 mM IPTG, 25 oC o/n	Ni	115	maybe
4	1796	pT7A3-MBP-(3C-TEV)-NRF2-6His, C-terminal 6His Tag	0.2 mM IPTG, 25 oC o/n	Ni	115	not sure
5	1800	pCold-6His-TEV-MBP, N-terminus His Tag	0.2 mM IPTG, 25 oC o/n	Ni	70	yes, but also truncated
6	1803	pCold-6His-GST-(E)-(3C)-PKARIIb, N-terminus His Tag	0.2 mM IPTG, 25 oC o/n	Ni	77	maybe, too many bands
7	1804	pCold-MBP-(a3C)-MCS-3C, C-terminal 6His Tag	0.2 mM IPTG, 25 oC o/n	Ni		can not be purified with Ni resin, vector itself will not translate to get C-6His tag
8	1809	pCold-MBP-(E3C)-FN3K, C-terminus his tag	0.2 mM IPTG, 25 oC o/n	Ni	80	no
9	1759	pCold-6H-(TEV)-MBP-(3C)-Keap1, N-6XHis	0.2 mM IPTG, 25 oC o/n	Ni	115	partial purified, mostly MBP, no MBP-Keap1
10	MBP-Keap1	partial purified, MBP-Keap1	0.1 mM IPTG, 25 oC o/n	Ni		no MBP-Keap1
11	1719	partial purified, pT7A3-MBP-(E3C)-NRF2, C-6His Tag	0.1 mM IPTG, 25 oC o/n	Ni	114	not pure
M		Fermentas PageRuler Plus