1737 2038Taq 2289

Aug11,2014

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Lane	Clone ID	Desc	Induction	Purification	MW, KDa	purification
1	1737	pT7A3E-His-TRX                          His-thioredoxin for making T7 DNA Polymerase complex	?mMIPTG, 25 oC o/n	Ni	5.72/13.4	8/8/2014 Manual run1 fraction4,5,6
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4	pageRulerPlusPrestained protein ladder
5	1737	pT7A3EHis-TRX                       His-thioredoxin for making T7 DNA Polymerase complex	?mMIPTG, 25 oC o/n	Ni ------dialyse	5.72/13.4	8/8/2014 Manual run1 fraction8,10
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7	2289	pT7A30-SMT3 SUMO tag Saccharomyces cerevisiae S288c SUMO family protein SMT3 (SMT3), C-His tag	?mMIPTG, 25 oC o/n	Ni ------dialyse Ni	5.42/14.1	8/8/2014 Manual run 9 fraction3,4,5
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10	1737	pT7A3EHis-TRX His-thioredoxin for making T7 DNA Polymerase complex	?mMIPTG, 25 oC o/n	Ni	5.72/13.4	8/9/2014 Manual run 4 fraction3,4,5
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Lane	Clone ID	Desc	Induction	Purification	MW, KDa	purification
1	1737	pT7A3EHis-TRX His-thioredoxin for making T7 DNA Polymerase complex	?mMIPTG, 25 oC o/n	Ni	5.72/13.4	8/9/2014 Manual run 4 fraction6
2	2038	L42-sso7d-KlenTaq	?mMIPTG, 25 oC o/n	Ni	7.02/70.1	8/11/2014 Manual run0 fraction9,10,11,12
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6	pageRulerPlusPrestained protein ladder
7	2038	L42-sso7d-KlenTaq	?mMIPTG, 25 oC o/n	Ni	7.02/70.1	8/11/2014 Manual run0 fraction13 8/10/2014 Manual run7 fraction11,12,13,14,19
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