1738 pEC-iMMLV-m

Cloning of 1738 pEC-iMMLV-m

Purpose: Cloning of 1738 pEC-iMMLV-m

Date: 2013-4-

Notebook:

Page:

Clone ID:1738

Experimenters: Shanyi

Results

cloning

Date:2013-3-

PCR use 1714 as template and primers

MMLV-EcoR1-Nde1-F + MMLV-W313F-R

MMLV-W313F-F + MMLV-L435G-R

MMLV-L435G-F + MMLV-Not1-Xho1-R

join 3 fragments by pcr using

MMLV-EcoR1-Nde1-F atcgac gaattc CAT ATG ACCCTAAATATAGAAGATGAGC

MMLV-Not1-Xho1-R (CTCCAGACACCTCTACCCTCCTC cat cac cat cac cat cac taa GCGGCCGC CTCGAG cgatca) tgatcgCTCGAGGCGGCCGCttagtgatggtgatggtgatgGAGGAGGGTAGAGGTGTCTGGAG

digest with  EcoR1 + Xho1

Colony Screening

Date: 2013

DIGEST WITH BamHI(ONLY ONE BAND)

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