1747 pT7A30-iMMLV
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Cloning of 1747 pT7A30-iMMLV
[edit]Purpose: Cloning of 1747 pT7A30-iMMLV
Date: May , 2013
Notebook:
Page:
Clone ID: 1747
Experimenters: Shanyi
Results
[edit]Cloning
[edit]subclone to 1727 pT7A30 using Nde1 + Xho1
digest 1738 with Nde1 + Xho1+DraIII+AlwN1,should get four bands(==>2000bp,==>1600bp,==>800bp,==>500bp),the 2000bp DNA is iMMLV
Colony screen
[edit]Cloning
[edit]subclone to 1727 pT7A30 using Nde1 + Xho1
digest 1738 with Nde1 + Xho1+BamHI,should get 3 bands,cut out the lower two bands.
Colony screen
[edit]