1773 pUltraBac-NRF2
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Cloning of1773 pUltraBac-NRF2
[edit]Purpose: Cloning of 1773 pUltraBac-NRF2
Date: 2013-7--2-
Notebook:05846
Page:
Clone ID:1773
Experimenters: Shanyi
Results
[edit]cloning
[edit]PCR using 1622 pLX304-NRF2 as template
http://www.excellgen.net/eln/images/1/16/1773pcr.JPG
digest PCR products with BamH1 + Xba1, then ligate to BamH1 + Xba1 digested 1246 pUltrabac
Colony Screening
[edit]Date: 2013-7-
Make miniprep and digest with Xho1 + BamH1
