1792-pT7A3-TthPol
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Cloning of 1792-pT7A3-TthPol
[edit]Purpose: Cloning of 1792-pT7A3-TthPol
Date: 2013-8-6--2013-8-8
Notebook:0
Page:
Clone ID:1792
Experimenters: yingying
Results
[edit]cloning
[edit]
| lane |
1 |
2 |
| 483,pET28b-TthPol |
1279, pT7A3 | |
| enzymes |
Nde1 + EcoR1 | |
Colony Screening
[edit]Date: 2013-8-9
Make miniprep and digest with Nde1 + EcoR1
Result intepretation/Summary
[edit]Conclusion
[edit]
Cloning of 1792-pT7A3-TthPol
[edit]Purpose: Cloning of 1792-pT7A3-TthPol
Date: 2013-8-13--2013-8-15
Notebook:0
Page:
Clone ID:1792
Experimenters: yingying
Results
[edit]cloning
[edit]
| lane |
1 |
2 |
| 483,pET28b-TthPol |
1279, pT7A3 | |
| enzymes |
Nde1 + EcoR1 | |
Colony Screening
[edit]Date: 2013-8-15
Make miniprep and digest with Nde1 + EcoR1
digest with Nde1 + BamH1
clone 1 for stock
Result intepretation/Summary
[edit]Conclusion
[edit]