1797 1498 1781 1796 1497 1542
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Protein Purification
[edit]2013/7/16
SHANYI
| Lane |
Name |
Protein Name |
Clone ID |
Host |
Purification |
pI/Mw |
Expression | |
| 1 |
pCold-6His-3C-MBP |
3C-MBP |
1797 |
BL21(DE3)Star, pRare2-pLysS |
autoinduce |
Ni beads |
62KD | |
| 2 |
no IPTG | |||||||
| 3 |
autoinduce |
|||||||
| 4 |
2hIPTGinduce | |||||||
| 5 |
pT7A3-TaqDNALigase |
TaqDNALigase |
1498 |
Nico21 DE3 plysN |
10 ml Ni column
Bio-Sil TSK 250 Q column |
76KD |
||
| 6 |
pTCold-MBP-(3C-TEV)-PKARIIb |
MBP-(3C-TEV)-PKARIIb |
1781 |
Nico21(DE3)pRare2pLysS |
Ni column Amylose column |
5.11 / 93127.81 |
||
| 7 |
pT7A3-MBP-(3C-TEV)-NRF2-6His |
NRF2 |
1796 |
Nico21(DE3)pRare2pLysS |
Ni column
Amylose column TEVdigestion |
4.67 / 68345.09 |
||
| 9 |
pT7A3-ToughTaq |
ToughTaq |
1497 |
Nico21 DE3 plysN |
0.2mM IPTG, 25c overnight |
Ni column |
72KD |
yes |
| 10 |
pT7A3-sso7d-Native-ToughTaq |
ToughTaq |
1542 |
BL21(DE3)Star, pRare2-pLysS |
0.2mM IPTG,
25c overnight
|
Ni column |
70KD |
yes |
| 11 |
pT7A3-ToughTaq |
ToughTaq |
1497 |
Nico21 DE3 plysN |
0.2mM IPTG,
37c ,4h |
Ni column |
72KD |
yes |
| 12 |
pT7A3-sso7d-Native-ToughTaq |
ToughTaq |
1542 |
BL21(DE3)Star, pRare2-pLysS |
0.2mM IPTG,
37c ,4h |
Ni column |
70KD |
yes |