1808-pT7A3-6His-NLS-Flpo
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Cloning of 1808-pT7A3-6His-NLS-Flpo
[edit]Purpose: Cloning of 1808-pT7A3-6His-NLS-Flpo
Date: 2013-8-2---2013-8-5
Notebook:0
Page:
Clone ID:1808
Experimenters: yingying
Results
[edit]cloning
[edit]
1520 pDIRE as template
Flpo-Nco1-F
Flpo-Xho1-R
| lane |
1 |
2 |
| PCR product |
1279 pT7A3 | |
| enzymes |
Nco1 + Xho1 | |
Colony Screening
[edit]Date: 2013-8-5
Make miniprep and digest with Nco1 + Xho1
Result intepretation/Summary
[edit]Conclusion
[edit]