1811-pT7A3-VPol1

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Cloning of 1811-pT7A3-VPol1

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Purpose: Cloning of 1811-pT7A3-VPol1

Date: 2013-9-11--2013-9-13

Notebook:0

Page:

Clone ID:1811

Experimenters: yingying

Results

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cloning

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subclone from 1793 pET28b-VPol1 using Nco1 + Xho1

lane
 1
 2
 3

 1793 pET28b-VPol1
 1279,pT7A3
 1780,pT7A3-T7-RNAPol-Y639F

                          Nco1 + Xho1


Colony Screening

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Date: 2013-9-13

Make miniprep and digest with Nco1 + Xho1


protein purification

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Result intepretation/Summary

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Conclusion

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