1813 1811
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September, 2013
| Desc |
CloneID |
Name | |
| 1 |
Fermentas PageRuler Plus |
||
| 2 |
"purified" Flp dialysed |
1813 |
FLP |
| 3 |
FLP Ni HisTrap HP eluate |
1813 |
FLP |
| 4 |
FLP Haparin HyperD Fraction 57 (1M NaCl elute) Nothing is eluted from 0.1 to 1 M NaCl |
1813 |
FLP |
| 5 |
50 mM arginine wash, 5 ml Ni HisTrap HP column |
1811 |
Vpol |
| 6 |
100 mM arginine wash, 5 ml Ni HisTrap HP column |
1811 |
Vpol |
| 7 |
150 mM arginine wash, 5 ml Ni HisTrap HP column |
1811 |
Vpol |
| 8 |
20 to 300 mM Imidazole gradient elute, fraction #6, 5 ml Ni HisTrap HP column |
1811 |
Vpol |
| 9 |
His-MBP-Taq-Muts contaminated by some FLP |
211 |
His-MBP-Taq-Muts |
| 10 |
His-MBP-Taq-Muts eluted from amylose beads |
211 |
His-MBP-Taq-Muts |