1819-pT7A30-TthDNALigase-C-6XHis

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Cloning of 1819-pT7A30-TthDNALigase-C-6XHis

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Purpose: Cloning of 1819-pT7A30-TthDNALigase-C-6XHis

Date: 2013-8-13---

Notebook:0

Page:

Clone ID:1819

Experimenters: yingying

Results

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cloning

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PCR the c-terminus fragment

template: 1035pET28b-Tth-Ligase-K294R

Tth-Ligase-Nco1-F

Tth-Ligase-HindIII-R



lane
 1
 2

 PCR product
 1818 pT7A30-TthDNALigase
enzymes
 Nco1 + HindIII



Colony Screening

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Date: 2013-8-21

Make miniprep and digest with Nco1 + HindIII/Xho1


Result intepretation/Summary

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Conclusion

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