1821 pT7A30-TthPol-C6XHis

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Cloning of 1821 pT7A30-TthPol-C6XHis

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Purpose: Cloning of 1821 pT7A30-TthPol-C6XHis

Date: 08, 14, 2013

Notebook: 

Page:

Clone ID: 1821

Experimenters: Shanyi

Results

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Cloning

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PCR from ATCC Genomic DNA from Thermus thermophilus Strain DSM 7039 [HB27] ATCC BAA-163D-5


digest with Nde1 + Not1


 Colony screen

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 make miniprep
digestion with NdeI+Nco1 will get a small fragment+ a large fragment


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Result intepretation/Summary

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Conclusion

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