1823 pCold-HTNCre
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Cloning of 1823 pCold-HTNCre
[edit]Purpose: Cloning of 1823 pCold-HTNCre
Date: 08, 12, 2013
Notebook:
Page:
Clone ID: 1823
Experimenters: Shanyi
Results
[edit]Cloning
[edit]subclone from 1708 pT7A3-HTNCre TO 1804 pCold-MBP-(E3C)using Nco1 + Xho1
Colony screen
[edit]8/13/2013: make miniprep, digest with NheI+BamHI
the parental vector 1708 does not have NheI site, 1804 should get a 1344bp band, 1823 should get a 614bp band
[edit]