1827 1828 1829 1830 1831

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Cloning of 1827 1828 1829 1830 1831

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Purpose: Cloning of 1827 1828 1829 1830 1831

Date: 08, 20, 2013

Notebook: 

Page:

Clone ID: 1827 1828 1829 1830 1831

Experimenters: Shanyi

Results

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Cloning

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1827 pT7A30-FN3K     subclone from 1822 pT7A3-MBP-(E3C)-FN3K TO 1727pT7A30

1828 pT7A3-8H-FN3K  subclone from 1822 pT7A3-MBP-(E3C)-FN3K TO 1725pT7A3-8H

1829 pCold-FN3K        subclone from 1757 pT7A3-MBP-(3C)-FN3K TO v19 pET28-cold

1830 pET28b-FN3K     subclone from 1757 pT7A3-MBP-(3C)-FN3K TO V12 pET28b

1831 pET30-FN3K       subclone from 1809 pCold-MBP-(E3C)-FN3K TO V15 pET30b


 Colony screen

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digest with Nde1 + Xho1 




Result intepretation/Summary

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Conclusion

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