1827 1828 1829 1830 1831 1823

Protein Expression

2013/8/22
SHANYI

Lane	Vector Name	Name	Clone ID	Host	Induction	Purification	pI/Mw	Expression
1	pT7A30-FN3K	FN3K	1827	5 ml, BL21DE3pRare2pLysS	0.2 mM IPTG    37oC,4h	Ni beads	6.95 / 36349.49
2					0.2 mM IPTG   RT o/n			low
3	pT7A3-8H-FN3K		1828	5 ml, BL21DE3pRare2pLysS	0.2 mM IPTG    37oC,4h	Ni beads	7.00 / 38090.41	low
4					0.2 mM IPTG   RT o/n			yes
5	pCold-FN3K		1829	5 ml, BL21DE3pRare2pLysS	0.2 mM IPTG  15oC,o/n	Ni beads	7.29 / 38063.47
6	pET28b-FN3K		1830	5 ml, BL21DE3pRare2pLysS	0.2 mM IPTG   37oC,4h	Ni beads	7.29 / 38794.21
7					0.2 mM IPTG   RT o/n
8	pET30b-FN3K		1831	5 ml, BL21DE3pRare2pLysS	0.2 mM IPTG   37oC,4h		6.95 / 36349.49
9					0.2 mM IPTG    RT o/n	Ni beads		low
10	PageRuler Plus protein ladder
11	pCold-HTNCre	HTNCre	1823	5 ml, Nico21(DE3)plysN	0.2 mM IPTG 15oc o/n	Ni beads	9.47 / 45756.94	YES
12				]		Ni column		YES