1837 pPinkα-HC-Enterokinase
Cloning of 1837 pPinkα-HC-Enterokinase
[edit]Purpose: Cloning of1837 pPinkα-HC-Enterokinase
Date: 2013-8-30--
Notebook:0
Page:
Clone ID:1837
Experimenters: Shanyi
Results
[edit]cloning
[edit]PCR from 1815 pMAL-c5E-Enterokinase
digest with FspI + Kpn1
ligate to StuI + Kpn1 digested vector pPinkα-HC
Colony Screening
[edit]Date: 2013-8-30
Make miniprep and digest with NdeI+KpnI--->675bp
2013-9-3
digest with Pst1+Kpn1, should be 300bp
Cloning of 1837 pPinkα-HC-Enterokinase
[edit]Purpose: Cloning of1837 pPinkα-HC-Enterokinase
Date: 2013-10-30--
Notebook:0
Page:
Clone ID:1837
Experimenters: Shanyi
Results
[edit]cloning
[edit]PCR from 1815 pMAL-c5E-Enterokinase
Enterokinase-Xho1-F2 tagctactcgagaaa agg attgtcggaggaagtgactc
Enterokinase-Kpn1-R tgctagGGTACCCTAgTGATGATGATGATGATGTAGAAAAC
digest with XhoI + Kpn1
ligate to XhoI + Kpn1 digested vector pPinkα-HC
Colony Screening
[edit]Date: 2013-11-4
Make miniprep and digest with XhoI+KpnI
