1838-pEC-hia5
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Cloning of 1838-pEC-hia5
[edit]Purpose: Cloning of 1838-pEC-hia5
Date: 2013-8-30--2013-9-4
Notebook:0
Page:
Clone ID:1838
Experimenters: shanyi
Results
[edit]cloning
[edit]
| lane |
1 |
2 |
| PCR product |
1454,pEC | |
| HindIII + BamH1 | ||
Colony Screening
[edit]Date: 2013-9-5
Make miniprep and digest with HindIII + BamH1
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]
Cloning of 1838-pEC-hia5
[edit]Purpose: Cloning of 1838-pEC-hia5
Date: 2013-9-4--2013-9-9
Notebook:0
Page:
Clone ID:1838
Experimenters: yingying
Results
[edit]cloning
[edit]
pcr1:Hia5-1 to Hia5-12
pcr2:Hia5-12 to Hia5-24
PCR:Hia5-1 to Hia5-24
| lane |
1 |
2 |
| PCR product |
1454,pEC | |
| HindIII + BamH1 | ||
Colony Screening
[edit]Date: 2013-9-9
Make miniprep and digest with HindIII + BamH1
Result intepretation/Summary
[edit]Conclusion
[edit]
fix clone1
2013-9-13
HindIII + BamH1
