1853-pET28b-yFLP
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Cloning of 1853-pET28b-yFLP
[edit]Purpose: Cloning of 1853-pET28b-yFLP
Date: 2013-10-16---2013-10-18
Notebook:0
Page:
Clone ID:1853
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR using 1857 pOG44 as template,
pcr 1:
FLP-HindIII-F
FLP-L33S-R
pcr 2:
FLP-L33S-F
FLP-Y108N-R
pcr 3:
FLP-Y108N-F
FLP-S294P-R
pcr 4:
FLP-S294P-F
FLP-Xho1-R
Join 4 fragments using
FLP-HindIII-F + FLP-Xho1-R
| lane |
1 |
2 |
| PCR |
375, pET28b-Cre | |
| HindIII + Xho1 | ||
Colony Screening
[edit]Date: 2013-10-18
Make miniprep and digest with Hind111+EcoR1
