1855 pEC2-yPolyA-pol
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Cloning of 1855 pEC2-yPolyA-pol
[edit]Purpose: Cloning of1855 pEC2-yPolyA-pol==
Date: 2014-7----
Notebook:0
Page:
Clone ID:1855
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from 2217 pDONR221-PAP1
digest with Nde1 + XhoI, clone to 2015
Colony Screening
[edit]Date: 2014_7
Make miniprep, use Nde1 + XhoI to find positive clone
PCR