1860 1860 1853 1518 1519 1919 1654 1901 1859 1859 1873 1873

1860_1860_1853_1518_1519_1919_1654_1901_1859_1859_1873_1873

PageRuler Plus 10, 15, 25(red), 35, 55, 70(red), 100, 130, 250

Induce at RT o/n 0.2mM IPTG

Lane	Name	Protein Name	Clone ID	Host	Induction	Purification	pI/Mw	Expression
1	pT7A3-yFLP	yFLP	1860	5 ml, Nico21(DE3)	0.2 mM IPTGRT o/n	Ni beads	9.76 / 52.8
2				5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads
3	pET28b-yFLP		1853	5 ml, T7 express	0.2 mM IPTG RT o/n	Ni beads
4	PageRuler Plus Prestained protein ladder
5	pET28b- T3-DNA-Ligase	T3-DNA-Ligase	1518	5 ml, T7 express	0.2 mM IPTG RT o/n	Ni beads	5.62 / 41.6	Y
6	pT7A3- T3-DNA-Ligase		1519	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.62 / 41.6	Y
7	pT7A3-6H-sso7d- T3-DNA-Ligase		1919	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.97 / 48.6	Y
8	pT7A3- T3-DNA-Ligase2	T3-DNA-Ligase2	1654	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.62 / 41.6	Y
9	pT7A3-6H-sso7d- T3-DNA-Ligase2		1901	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.97 / 48.6	Y
10	pT7A3-TNF9	TNF9	1859	5 ml, Nico21(DE3)pRare2pLysS	0.2 mM IPTG RT o/n	Ni beads	26.6
11				5 ml, TunerDE3pRare2pLysS	0.2mM IPTG RT o/n	Ni beads
12	pT7A30-IL21	IL21	1873	5 ml, Nico21(DE3)pRare2pLysS	0.2 mM IPTG RT o/n	Ni beads	17.3
13				5 ml, TunerDE3pRare2pLysS	0.2 mM IPTG RT o/n	Ni beads