1888-pCMV5-Gag-TagGFP-WPRE-oriP

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Cloning of 1888-pCMV5-Gag-TagGFP-WPRE-oriP

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Purpose: Cloning of 1888-pCMV5-Gag-TagGFP-WPRE-oriP

Date: 2013-10-23---2013-10-25

Notebook:0

Page:

Clone ID:1888

Experimenters: yingying

Results

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cloning

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PCR using 109 pET28b-TagGFP as template
TagGFP-EcoR1-F
TagGFP-Eag1-R


PCR product
 1449 pCMV5-Gag-NoStop-WPRE-oriP

     EcoR1 + Eag1


Colony Screening

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Date: 2013-10-25

Make miniprep and digest with EcoR1 + Eag1




Result intepretation/Summary

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Conclusion

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