1891-pET28b-TAB5
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Cloning of 1891-pET28b-TAB5
[edit]Purpose: Cloning of 1891-pET28b-TAB5
Date: 2013-10-29----2013-11-1
Notebook:0
Page:
Clone ID:1891
Experimenters: yingying
Results
[edit]cloning
[edit]
gene synthesis
pcr1:TAP5-1 to TAP5-14
pcr2:TAP5-15 to TAP5-28
PCR: TAP5-1 to TAP5-28
| lane |
3 |
4 |
| PCR product |
1225,pET28b-T7-Endo1 | |
| Nde1 + Xho1 | ||
Colony Screening
[edit]Date: 2013-11-1
Make miniprep and digest with Nde1 + Xho1
Result intepretation/Summary
[edit]Conclusion
[edit]