1899-pPinkalpha-HC-HTNCre
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Cloning of 1899-pPinkalpha-HC-HTNCre
[edit]Purpose: Cloning of 1899-pPinkalpha-HC-HTNCre
Date: 2013-10-28--2013-10-31
Notebook:0
Page:
Clone ID:1899
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR using 298HTNCre as template
Cre-Kpn1-R
6XHis-Xho1-F2
| lane |
7 |
8 |
| PCR product |
215 pPinkalpha-HC | |
| Xho1 + Kpn1 | ||
Colony Screening
[edit]Date: 2013-10-31
Make miniprep and digest with Xho1 + Kpn1
digest with BamH1
Result intepretation/Summary
[edit]Conclusion
[edit]