1900 pT7A3-6H-sso7d-T4-DNA-Ligase

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Cloning of 1900 pT7A3-6H-sso7d-T4-DNA-Ligase

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Purpose: Cloning of 1900 pT7A3-6H-sso7d-T4-DNA-Ligase

Date: 2013-10-22---

Notebook:0

Page:

Clone ID:1900

Experimenters:  shanyi

Results

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CLONING

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subclone T4 DNA ligase from 240 pET28b-T4-Ligase OR 1483 pT7A3-T4_DNA_Ligase to 1846 pT7A3-6H-sso7d using Nde1 + Xho1



Colony Screening

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Date: 2013-10-24

Make miniprep, digest with Nco1 + Nde1 + Xho1--> T4 DNAligase + sso7d bands



stock 1900-3


Result intepretation/Summary

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Conclusion

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