1901 pT7A3-6H-sso7d-T3-DNA-Ligase-2

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Cloning of 1901 pT7A3-6H-sso7d-T3-DNA-Ligase-2

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Purpose: Cloning of 1901 pT7A3-6H-sso7d-T3-DNA-Ligase-2

Date: 2013-10-22---

Notebook:0

Page:

Clone ID:1901

Experimenters:  shanyi

Results

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CLONING

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subclone T3 DNA ligase from 1654 pT7A3-T3 DNA ligase to 1846 pT7A3-6H-sso7d using Nde1 + Xho1



Colony Screening

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Date: 2013-10-24

Make miniprep, digest with Nco1 + Nde1 + Xho1--> T3 DNAligase + sso7d bands



stock 1901-3


Result intepretation/Summary

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Conclusion

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