1904 pT7A3-T3-DNA-Ligase-sso7d

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Cloning of 1904 pT7A3-T3-DNA-Ligase-sso7de

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Purpose: Cloning of 1904 pT7A3-T3-DNA-Ligase-sso7d

Date: 2013-10-22---

Notebook:0

Page:

Clone ID:1903

Experimenters:  shanyi

Results

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CLONING

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subclone T3 DNA ligase from 1096 pET28b-T3-Ligase or 1500 pT7A3-T3-ligase to 1847 pT7A30-sso7d-6H using Nde1 + Xho1



Colony Screening

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Date: 2013-10-24

Make miniprep, digest with Nde1 + Xho1--> T3 DNAligase + sso7d bands

File:1904DIGET.JPG


stock 1904-1


Result intepretation/Summary

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Conclusion

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