1922-pLVX-Kozak-FRT-TurboRFP-ODC-2Stop-FRT-TurboGFP
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Cloning of 1922-pLVX-Kozak-FRT-TurboRFP-ODC-2Stop-FRT-TurboGFP
[edit]Purpose: Cloning of 1922-pLVX-Kozak-FRT-TurboRFP-ODC-2Stop-FRT-TurboGFP
Date: 2013-11-14---2013-11-18
Notebook:0
Page:
Clone ID:1922
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR1 BGH terminator from 1522 CMV-Brainbow-2.1-R using
BGHpA-EcoR1-F
BGHpA-hGHpA-R
PCR2 hGH terminator from 1444 pLVX-Kozak-FRT-TurboRFP-ODC-FRT-TurboGFP using
BGHpA-hGHpA-F
FRT-hGHpA-Xho1-R
BGHpA-EcoR1-F+FRT-hGHpA-Xho1-R
| Lane |
1 |
2 |
| PCR product |
1444 pLVX-Kozak-FRT-TurboRFP-ODC-FRT-TurboGFP | |
| EcoR1 + Xho1 | ||
Colony Screening
[edit]Date: 2013-11-18
Make miniprep and digest with EcoR1 + Xho1
digest with EcoR1 + BamH1
digest with Xho1 + Xba1
digest with EcoR1 + Xho1