1927 1928 1930 1873 1941 1949 1987 1988 1860 1860 cre

Protein Expression

2013/12/20
SHANYI

Lane	Name	Protein Name	Clone ID	Host	Induction	Purification	pI/Mw	express
1	pT7A3-T4-DNA-Ligase-sso7d-6H	T4-DNA-Ligase	1927	5 ml, Nico21(DE3)plysN	0.5 mM IPTG  37oC 2h	Ni beads	7.57 / 64246.86
2	pT7A3-T7-DNA-Ligase-sso7d-6H	T7-DNA-Ligase	1928	5 ml, Nico21(DE3)plysN	0.5 mM IPTG 37oC 2h	Ni beads	5.97 / 50.1
3	pT7A3-T3-DNA-Ligase-sso7d-6H	T3-DNA-Ligase	1930	5 ml, Nico21(DE3)plysN	0.5 mM IPTG 37oC 2h	Ni beads	5.84 / 48.3
4	pT7A30-IL21	IL21	1873	5 ml, Nico21(DE3)pRare2pLysS	0.5 mM IPTG 37oC 2h	Ni beads	8.6/17.3
5	pT7A3-HC-6H-TNF9	TNF9	1941	5 ml, Nico21(DE3)pRare2pLysS	0.5 mM IPTG 37oC 2h	Ni beads	6.87 / 21.7
6	pT3A3-Kfu-G590S-sso7d	Kfu	1949	5 ml, Nico21(DE3)plysN	0.5 mM IPTG 37oC 2h	Ni beads	7.87 / 99.3
7	pT7A3-TAT-MOD	TAT-MOD	1987	5 ml, BL21(DE3) Star pRare2pLysS	0.5 mM IPTG 37oC 2h	Ni beads	9.29 / 36.4
8	pT7A3-LacI-SV40	LacI-SV40	1988	5 ml, BL21(DE3) Star pRare2pLysS	0.5 mM IPTG 37oC 2h	Ni beads	8.68 / 40.9
9	PageRuler Plus Prestained protein ladder
10	pT7A3-yFLP	yFLP	1860	5 ml, cag629	0.5 mM IPTG 37oC 2h	Ni beads	9.76 / 52.8
11			1860	5 ml, cag629	0.5 mM IPTG 37oC 2h	Ni beads
12	pT7A3-HTNCre	HTNCre	1708	5 ml, Nico21(DE3)plysN	0.5 mM IPTG 37oC 2h