1937 pT7A3E-DNA-Pol-1

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Cloning of 1937 pT7A3E-DNA-Pol-1

Purpose: Cloning of1937 pT7A3E-DNA-Pol-1

Date: 2014-1----

Notebook:0

Page:

Clone ID:1937

Experimenters: shanyi

Results

subcloning

subclone E Coli DNA Polymerase I from 1141 to 1892 pT7A3E,using Nde1 + HindIII

Colony Screening

Date: 2013-10-23

Make miniprep, digest with NdeI+XhoI, will get four bands=>500,750, 1500,vector

Result intepretation/Summary

Conclusion

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Cloning