1944 pT7A3E-polyAPol

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Cloning of 1944 pT7A3E-polyAPol

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Purpose: Cloning of 1944 pT7A3E-polyAPol==

Date: 2014-1----

Notebook:0

Page:

Clone ID:1944

Experimenters:  shanyi

Results

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subcloning

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subclone E coli poly(A) polymerase I from 1140 to 1892 pT7A3E ,using NdeI+XhoI


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Colony Screening

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Date: 2013-10-23

Make miniprep, digest with NdeI+XhoI

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Subcloning and Screening

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Digested by Nde1 and Xho1

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1944 PCR product 4/23/2015

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1944 Digestion by Nde1+Xho1  4/28/2015

Result intepretation/Summary

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Conclusion

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