1948 1950 1993 1994 1998 2019 2023 2037 2220
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Protein Expression
[edit]2014/5/30
SHANYI
| Lane |
Name |
Protein Name |
Clone ID |
Host |
Induction |
SAMPLE |
pI/Mw |
express |
| 1 |
pT7A3E-IPP |
inorganic pyrophosphatase |
1948 |
15 ml, Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
5.58/22.3 |
not sure |
| 2 |
pT7A3E-Fpg |
MutM |
1950 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
8.47/31.9 |
yes |
| 3 |
pT7A3-Endonuclease-VIII |
Endonuclease-VIII |
1993 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
S |
8.34/32 |
yes |
| 4 |
pT7A3E-Exo-III |
Exo-III |
1994 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
6.1/32.7 |
yes |
| 5 |
pT7A3E-Ecoli_RecA |
Ecoli_RecA |
1998 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
5.42/39.7 |
yes |
| 6 |
pT7A3E-Ecoli_RecA |
Ecoli_RecA |
|
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N magnetic beads |
s |
5.42/39.7 |
yes |
| 7 |
pageRuler Plus Prestained protein ladder |
|
||||||
| 8 |
pT7A3E-Endo-III |
Endo-III |
2019/2058 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
8.14/25.3 |
YES, cell lysed during IPTG induction |
| 9 |
pT7A3E-ExcellScript |
MMLV superscript III |
2023 |
15 ml, Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
8.92/77.7 |
yes |
| 10 |
pLC6H-Dpo4 |
error‐prone family Y |
2037 |
15 ml,
Tuner(DE3)plysN |
0.3mM IPTG RT O/N |
S |
9.19/40.8 |
not sure |
| 11 |
pMal-p-MBP-Tat-NLS-iFLP-6H |
MBP-Tat-NLS-iFLP-6H |
2220 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
S |
96 |
no |
| 12 |
2220 |
15 ml,
Tuner(DE3)plysN |
s |
no |