1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP

From ELN
Jump to navigation Jump to search

Cloning of 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriPP

[edit]

Purpose: Cloning of 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP

Date: 2013-12-15---

Notebook:0

Page:

Clone ID:1968

Experimenters:  shanyi

Results

[edit]

cloning

[edit]

subclone NLS-Cre from 1400 pCMV5-Gag-NLS-Cre-WPRE-oriP to 1969 pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP or 1970 pCMV5-Gag-p2b-TagGFP-WPRE-oriP

USING EcoR1+Eag1



Colony Screening

[edit]

Date: 2013-12-5

Make miniprep, digest with EcoR1 +EagI






Result intepretation/Summary

[edit]

Conclusion

[edit]
Retrieved from "https://eln.excellgen.com/index.php?title=1968_pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP&oldid=10818"