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Protein Expression

2014/7/24
SHANYI

Lane	Name	Protein Name	Clone ID	Host	Induction	Purification	pI/Mw	express	sample
1	pT7A3E-Ecoli-Endo-v		2018	5 ml, Nico21(DE3)plysN	0.2 mM IPTG  RT O/N	Ni beads	7.82/26.4	yes	media
2	pT7A3-Fast-Kfu		2028	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT O/N	Ni beads	7.87/99.3	yes
3	pT7A3E-Pfu-RNaseH2		2036	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT O/N	Ni beads	9.35/27	yes
4	pT7A30-wt-Taq-sso7d		2080	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT O/N	Ni beads	6.24/104.1
5	PageRuler Plus Prestained protein ladder
6	pT7A3-MBP-(TEV)-MBP-NusA-sso7d-6H		2093	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	Ni beads	4.89/148.6	yes
7	pT7A3E-ULP1		2210	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	Ni beads	6.59/27.3	?
8	pT7A3E-PDI1		2212	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	Ni beads	4.49/59.3
9	pT7A3E-T4-GP32		2242	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	Ni beads	5.19/35.2
10	pT7A3E-pelB-Lambda-Exo		2244	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	Ni beads	6/29.3
11	pT7A3E-pelB-Lambda-Exo		2244	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	Ni beads	6/29.3		media
12	pT7A3E-OmpA-Lambda-Exo		2245	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT O/N	Ni beads	6.13/29.2