2018 pT7A3E-Ecoli-Endo-V

Cloning of 2018 pT7A3E-Ecoli-Endo-V

Purpose: Cloning of2018 pT7A3E-Ecoli-Endo-V

Date: 2014-1----

Notebook:0

Page:

Clone ID:12018

Experimenters:  shanyi

Results

cloning

PCR from E coli genomic DNA
Ecoli-Endo-V-Nde1-F
atcgac catatggatctcgcgtcattacgcgct
Ecoli-Endo-V-Xho1-R
(tgcgctatacagcaaatcagccc ctcgag tagcta)
tagctactcgaggggctgatttgctgtatagcgca

clone  into Nde1 +Xho1 digested 1963 pT7A3E-HC  using Nde1 +Xho1

Colony Screening

Date: 2013-10-23

Make miniprep, digest with NdeI+XHOI

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