2035-pLN6H-Tth-RNase-H

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Cloning of 2035-pLN6H-Tth-RNase-H

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Purpose: Cloning of 2035-pLN6H-Tth-RNase-H

Date: 2014-2-12---2014-2-14

Notebook:0

Page:

Clone ID:2035

Experimenters: yingying

Results

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cloning

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PCR from Thermus thermophilus HB8 genomic DNA (-80 oC ATCC box)
Tth-RNaseH-Nde1-F
Tth-RNaseH-Xho1-R

lane
5
6

pcr
2029 pLNC6H

Nde1 + Xho1


Colony Screening

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Date: 2014-2-14

Make miniprep and digest with Nde1 + Xho1


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Result intepretation/Summary

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Conclusion

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