2041 pT7A3E-HC-sfGFP

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Cloning of 2041 pT7A3E-HC-sfGFP

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Purpose: Cloning of 2041 pT7A3E-HC-sfGFP==

Date: 2014-1----

Notebook:0

Page:

Clone ID:2041

Experimenters:  shanyi

Results

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subcloning

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subclone sfGFP from 1565or 2027 to  1963 pT7A3E-HC ,using Nhe1 + HindIII




Colony Screening

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Date: 2013-10-23

KAN-->AMP, E coli colonies are green under blue light transilluminator

Make miniprep, digest with Nhe1 + HindIII





Result intepretation/Summary

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Conclusion

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