2042 pT7A3E-HC-sfGFP
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Cloning of 2042 pT7A3E-HC-sfGFP
[edit]Purpose: Cloning of 2042 pT7A3E-HC-sfGFP==
Date: 2014-1----
Notebook:0
Page:
Clone ID:2042
Experimenters: shanyi
Results
[edit]subcloning
[edit]subclone sfGFP from 1565 to 1279 pT7A3 ,using Nhe1 + HindIII
Colony Screening
[edit]Date: 2013-10-23
Make miniprep, digest with Nhe1 + HindIII
DIGEST WITH XbaI+NheI, should get a small band=>100bp