2047-pCold-C6H
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Cloning of 2047-pCold-C6H
[edit]Purpose: Cloning of 2047-pCold-C6H
Date: 2014-2-4--2014-2-7
Notebook:0
Page:
Clone ID:2047
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR cold promoter from cold vector such as v19 pET28-cold
Cold-BglII-F
Cold-EcoR1-R2
| lane |
5 |
6 |
| pcr |
1892 pT7A3E | |
| BglII + EcoR1 | ||
Colony Screening
[edit]Date: 2014-2-7
Make miniprep and digest with EcoRV+Xba1/BglII + EcoR1
c:1892 pT7A3E
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]