2064 pT7A3E-NpuDnaE-C1A-ChiBD-6H

From ELN
Jump to navigation Jump to search

Cloning of 2064 pT7A3E-NpuDnaE-C1A-ChiBD-6H

[edit]

Purpose: Cloning of2064 pT7A3E-NpuDnaE-C1A-ChiBD-6H==

Date: 2014-2----

Notebook:0

Page:

Clone ID:2064

Experimenters:  shanyi

Results

[edit]

CLONING

[edit]

PCR from 1742 p8H-ELK-IN
NpuDnaE-N-C1A-Nde1-F E3 CGTTAA CATATG GCg TTAAGCTATGAAACGGAAATcTTG

NpuDnaE-N-Kpn1-R E5 gtcgtaggtaccATTCGGCAAATTATCAAC



digest with Nde1 +Kpn1



Colony Screening

[edit]

Date: 2014

Make miniprep, digest with Nde1 +Kpn1


Result intepretation/Summary

[edit]

Conclusion

[edit]
Retrieved from "https://eln.excellgen.com/index.php?title=2064_pT7A3E-NpuDnaE-C1A-ChiBD-6H&oldid=11399"