2069-pEC-ha-Plac-hia5
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Cloning of 2069-pEC-ha-Plac-hia5
[edit]Purpose: Cloning of 2069-pEC-ha-Plac-hia5
Date: 2014--2-4---2014-2-7
Notebook:0
Page:
Clone ID:2069
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR using these two oligos using 1838 pEC-hia5
ha-constitutive-PLac-F A3
ha-constitutive-PLac-Hia5-F
| lane |
1 |
2 |
| pcr |
1454 pEC | |
| HindIII + Xho1 | ||
Colony Screening
[edit]Date: 2014-2-7
Make miniprep and digest with HindIII + Xho1
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]