2074-pET28b-Penetratin-Cre
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Cloning of 2074-pET28b-Penetratin-Cre
[edit]Purpose: Cloning of 2074-pET28b-Penetratin-Cre
Date: 2014-2-21---2-14-2-24
Notebook:0
Page:
Clone ID:2074
Experimenters: yingying
Results
[edit]cloning
[edit]
| 2 | |
| 375 pET28b-Cre | |
| Nco1+ HindIII |
ligate to annealed oligos
Penetratin-Nco1-HindIII-T A2 catggcgcatcatcatcatcaccatCGTcagatcaaaATttggttccaaaaccggcgcatgaaatggaagaaa
Penetratin-Nco1-HindIII-B
Colony Screening
[edit]Date: 2014-1-24
Make miniprep and digest with Nco1+ Xho1
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]