2078-pEC2-DNA-Ladder

Cloning of 2078-pEC2-DNA-Ladder

Purpose: Cloning of 2078-pEC2-DNA-Ladder

Date: 2014-3-2---2014-3-7

Notebook:0

Page:

Clone ID:2078

Experimenters: yingying

Results

cloning

pcr1 using lambda DNA as template

Fragment 1: 370 bp
1 ul of 10 uM mXho1-BamH1-R
1 ul of 1 uM DNA-ladder-F
1 ul of 10 uM DNA-ladder-R1

 

pcr2 using lambda DNA as template

Fragment 2: 799 bp
DNA-ladder-F1
DNA-ladder-R2

pcr3 using lambda DNA as template

Fragment 3: 737 bp
DNA-ladder-F2
CTCATCACCCCCAAGTCTGGC AAGCTT TGAATGCCTGGAATTAATCAC
DNA-ladder-Xho1-R

PCR:pcr1+pcr2_pcr3

lane	5	6
	PCR	2015 pEC2
	BamH1 + Xho1

colony Screening

Date: 2013-1-24

Make miniprep and digest with Hind111

lane	1	2	3
	2078-1
	BamH1	Hind111	BamH1/Hind111 mix 1 and 2

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