2083 pFastbac1-p53
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Cloning of 2083 pFastbac1-p53
[edit]Purpose: Cloning of2083 pFastbac1-p53==
Date: 2014-2----
Notebook:0
Page:
Clone ID:2083
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from1341
p53-EcoR1-Nde1-F atcgac gaattc gcc acc cat ATGGAGGAGCCGCAGTCAGATC
p53-Xho1-6H-HindIII-R
(GACAGAAGGGCCTGACTCAGAC ctcgag CAT CAT CAT CAT CAT CAC TGA AAGCTT tactac)
gtagtaAAGCTTTCAGTGATGATGATGATGATGctcgagGTCTGAGTCAGGCCCTTCTGTC
digest with EcoR1 + HindIII
Colony Screening
[edit]Date: 2014
Make miniprep, digest with EcoR1 + HindIII
sent 2083-2 to sequence,