2106 pET28b-iFLP
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Cloning of 2106 pET28b-iFLP
[edit]Purpose: Cloning of 2106 pET28b-iFLP==
Date: 2014-3----
Notebook:0
Page:
Clone ID:2106
Experimenters: shanyi
Results
[edit]CLONING
[edit]subclone from 2114 pEC-iFLP to 1853 pET28b-yFLP using HindIII + Xho1
Colony Screening
[edit]Date: 2014
Make miniprep, digest with NdeI + HindIII + Xho1,there is no NdeI site in new FLP
Result intepretation/Summary
[edit]Conclusion
[edit]Protein Expression
[edit]
| Lane | ID | Protein Name | MW, pI | Tag | Host | Condition | Expression |
| 1 | 927 | pET28b-Cid1 |
42 | His | Nico21(DE3), pLysN | 15 ml, RM , 0.3M IPTG induce o/n | Yes |
| 2 | 3097 | pET28b-pQbeta |
no | Nico21(DE3), pLysN |
15 ml, RM , 0.3M IPTG RM induce o/n |
do not know | |
| 3 | 3098 | pET28b-AP205 |
no | Nico21(DE3), pLysN |
15 ml, RM , 0.3M IPTG RM induce o/n |
do not know | |
| 4 | 3074 | pET28b-HA | no | Nico21(DE3), pLysN |
15 ml, RM , 0.3M IPTG RM induce o/n |
do not know | |
| 5 | 3105 | pET28b-AaLS |
22 | His | Nico21(DE3), pLysN |
15 ml, RM , 0.3M IPTG RM induce o/n |
Yes |
| 6 | M | 198, 98(Y), 62, 49, 38, 28, 17(Red), 14, 6, 3 | |||||
| 7 | 2106 | pET28b-iFLP, KJE7 | 9.76 / 52.8 |
N-His | Tuner(DE3) |
15 ml, Rm, 0.3M IPTG RM induce o/n | |
| 8 | 2115 | pT7A3-iFLP, KJE7 |
9.76 / 52.8 |
N-His |
Tuner(DE3) |
15 ml, Rm, 0.3M IPTG RM induce o/n |
|
| 9 | 2106 | pET28b-iFLP, KJE8 |
9.76 / 52.8 |
N-His |
Tuner(DE3) | 15 ml, Rm, 0.3M IPTG RM induce o/n |
|
| 10 | 2115 | pT7A3-iFLP, KJE8 |
9.76 / 52.8 |
N-His |
Tuner(DE3) |
15 ml, Rm, 0.3M IPTG RM induce o/n |
|
| 11 | 2106 | pET28b-iFLP, pLysN |
9.76 / 52.8 |
N-His |
Nico21(DE3), pLysN |
15 ml, Rm, 0.3M IPTG RM induce o/n |
|
| 12 | 2115 | pT7A3-iFLP, pLysN |
9.76 / 52.8 |
N-His |
Nico21(DE3), pLysN |
15 ml, Rm, 0.3M IPTG RM induce o/n |
|
