2107 1836 2038 2040 2080 2178 2179 2184 2082 2086 2087 2088

Protein Expression

20134/5/7
SHANYI

Lane	Name	Protein Name	Clone ID	Host	Induction	Purification	pI/Mw		sample	express
1	pT7A3E-HC-Taq-Muts-6H	Taq-Muts-6H	2107	15 ml, NEB shuffle	0.5 mM IPTG  RT O/N	Ni beads	5.67/92.8		S
2				15 ml, Nico21(DE3)plysN		Ni beads			P	YES
3						Ni beads			S	YES
4						Ni beads			S	YES
5	PageRuler Plus Prestained protein ladder
6	pCold-MBP-Taq-Muts-6His	MBP-Taq-Muts-6His	1836	15 ml, NEB shuffle	0.5 mM IPTG RT O/N	Ni beads	5.38/135.8		S
7						Ni beads			P	YES
8				15 ml, Nico21(DE3)plysN		Ni beads			S
9						Ni beads			P	YES
10	pL42-sso7d-KlenTaq	sso7d-KlenTaq	2038	15 ml, Nico21(DE3)plysN		Ni beads			S	YES
11	pT7A3-6H-sso7d-wt-Taq	6H-sso7d-wt-Taq	2040	15 ml, Nico21(DE3)plysN		Ni beads			S
12	pT7A30-wt-Taq-sso7d	wt-Taq-sso7d	2080	15 ml, Nico21(DE3)plysN		Ni beads			S

Lane	Name	Protein Name	Clone ID	Host	Induction	SAMPLE	pI/Mw	express
1	pT7A3HC-6H-Tat-sc-MS2-iCre	6H-Tat-sc-MS2	2178	15 ml, Nico21(DE3)plysN	0.5 mM IPTG  RT O/N	S	30.5
2						P
3	pT7A3HC-6H-Tat-sc-MS2-Flpo	6H-Tat-sc-MS2	2179			S
4						P		YES
5	pMAL-c5E-R9-HA-iFlp	c5E-R9-HA-iFlp	2184			S	8.87/98
6						P		YES
7	PageRuler Plus Prestained protein ladder
8	pT7A30-TRX-sso7d-6H	TRX-sso7d-6H	2082	15 ml, Nico21(DE3)plysN	0.5 mM IPTG RT O/N	S	20	YES
9						P	20	YES
10	pT7A3-MBP-NusA-6H		2086			S	100	YES
11	pT7A3-MBP-sso7d-6H		2087			S	50	YES
12	pT7A3-GST-sso7d-6H		2088			S	35	YES